SIMPLE AND MILD PREPARATION OF AN ENZYME-IMMOBILIZED MEMBRANE FOR A BIOSENSOR USING BETA-TYPE CRYSTALLINE CHITIN

被引：16
|
作者：
OHASHI, E
KORIYAMA, T
机构：
[1] Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo, 192, 559-6, Kitano
来源：
ANALYTICA CHIMICA ACTA | 1992年 / 262卷 / 01期
关键词：
AMPEROMETRY; BIOSENSORS; CHITIN; ENZYME ELECTRODE; GLUCOSE;
D O I：
10.1016/0003-2670(92)80003-P
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A thin enzyme-immobilized membrane was prepared from squid pen chitin under simple and mild conditions. Squid pen chitin had better physical properties than crab chitin owing to the beta-type crystalline structure in the former. The membrane exhibited not only the ability to entrap enzyme molecules but also sufficient oxygen and glucose perm abilities useful for biosensors. A glucose sensor, which was constructed with glucose oxidase immobilized on the chitin membrane and an oxygen electrode, exhibited a wide range of linearity (0.125-2.0 mM) and possessed good stability.
引用
收藏
页码：19 / 25
页数：7
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