SEPARATION AND CHARACTERIZATION OF TETROL METABOLITES OF BENZO[A]PYRENE-DNA ADDUCTS USING HPLC AND SOLID-MATRIX ROOM-TEMPERATURE LUMINESCENCE

被引：10

作者：

TJIOE, SW

HURTUBISE, RJ

机构：

[1] Department of Chemistry, University of Wyoming, Laramie

来源：

TALANTA | 1995年 / 42卷 / 01期

关键词：

D O I：

10.1016/0039-9140(94)00216-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Four tetrols of benzo[a]pyrene-DNA adducts were separated using reversed-phase high performance liquid chromatography. Chromatographic fractions containing a given tetrol were readily characterized with solid-matrix room temperature luminescence techniques. Solid-matrix fluorescence and phosphorescence spectra at picogram amounts of a tetrol were easily obtained. Tetrol fractions collected based on retention time, but with no response from the ultraviolet detector, were characterized by solid-matrix luminescence spectra at very small quantities.

引用

页码：59 / 64

页数：6

共 23 条

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