A ONE-STEP MONOCLONAL-ANTIBODY TYPING PROCEDURE THAT SIMPLIFIES HLA CLASS-I AND CLASS-II TYPING

被引：8

作者：

LEE, JH ^{[1
]}

LIAS, M ^{[1
]}

DENG, CT ^{[1
]}

ETESSAMI, S ^{[1
]}

LOON, J ^{[1
]}

CHEN, M ^{[1
]}

BANH, L ^{[1
]}

CONGER, N ^{[1
]}

CONNORS, D ^{[1
]}

MANZO, A ^{[1
]}

AYOUB, G ^{[1
]}

TERASAKI, PI ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT SURG,LOS ANGELES,CA 90024

来源：

TISSUE ANTIGENS | 1994年 / 44卷 / 01期

关键词：

MHC; HLA TYPING; MONOCLONAL ANTIBODY; COMPLEMENT; CLASS-I; CLASS-II; ONE-STEP TYPING;

D O I：

10.1111/j.1399-0039.1994.tb02354.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed monoclonal antibodies to most HLA specificities, making it possible for us to devise a simple, rapid, one-step microcytotoxicity test. The test is performed by adding 1 mul of cells to 1 mul of antibody-complement mixture predotted on the microtest tray. The reactions are read following a 1-hour incubation period (30 minutes in some instances). The analysis of reactions seen on testing 105 class I antibodies and 50 class II antibodies is shown. A comparison of typing by the standard NIH method and the new one-step procedure showed a > 96% concordance in the 500 T cells and 200 B cells we examined. Class I and class II typing could be performed using B cells, thus obviating the need to isolate both T and B cells for HLA typing.

引用

页码：34 / 42

页数：9

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