ENHANCING EFFECT OF PLATELET-DERIVED GROWTH-FACTORS ON MIGRATION OF CORNEAL ENDOTHELIAL-CELLS

被引：12

作者：

IGUCHI, I ^{[1
]}

KAMIYAMA, K ^{[1
]}

WANG, XG ^{[1
]}

KITA, M ^{[1
]}

IMANISHI, J ^{[1
]}

YAMAGUCHI, N ^{[1
]}

SOTOZONO, C ^{[1
]}

KINOSHITA, S ^{[1
]}

机构：

[1] KYOTO PREFECTURAL UNIV MED,DEPT MICROBIOL,KAMIKYO KU,KYOTO 602,JAPAN

来源：

CORNEA | 1995年 / 14卷 / 04期

关键词：

PLATELET-DERIVED GROWTH FACTOR; ENDOTHELIAL CELLS; CHEMOTACTIC MIGRATION; WOUND HEALING;

D O I：

10.1097/00003226-199507000-00004

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

To investigate the role of platelet-derived growth factor (PDGF) in corneal wound healing, we examined the effect of human natural PDGF, recombinant PDGF-BB, and PDGF-AA on the migration of rabbit corneal endothelial cells. In a modified Boyden chamber in fetal bovine serum-free conditions, natural PDGF and PDGF-BB, at a concentration of 1-3 or 10 ng/ml, enhanced the migration of endothelial cells, whereas at a higher concentration (10 or 30 ng/ml), this enhanced migration was suppressed; the optimal concentration range for enhancing migration was 3-10 ng/ml), this enhanced migration was suppressed; the optimal concentration range for enhancing the migration. Natural PDGF and PDGF-BB activity was found for up to 6 h after the beginning of culture, and was completely blocked by anti-PDGF neutralizing antibodies. A checkerboard assay demonstrated that PDGF-BB had a chemotactic effect on the corneal endothelial cell migration. These results suggest that natural PDGF and PDGF-BB, but not PDGF-AA, are involved in corneal wound healing by stimulating the migration of corneal endothelial cells.

引用

页码：365 / 371

页数：7