THE THROMBIN RECEPTOR IN HUMAN PLATELETS IS COUPLED TO A GTP-BINDING PROTEIN OF THE G-ALPHA(Q) FAMILY

被引：39

作者：

BENKA, ML

LEE, M

WANG, GR

BUCKMAN, S

BURLACU, A

COLE, L

DEPINA, A

DIAS, P

GRANGER, A

GRANT, B

HAYWARDLESTER, A

KARKI, S

MANN, S

MARCU, O

NUSSENZWEIG, A

PIEPENHAGEN, P

RAJE, M

ROEGIERS, F

RYBAK, S

SALIC, A

SMITHHALL, J

WATERS, J

YAMAMOTO, N

YANOWITZ, J

YEOW, K

BUSA, WB

MENDELSOHN, ME

机构：

[1] TUFTS UNIV, NEW ENGLAND MED CTR, MOLEC CARDIOL RES CTR, BOSTON, MA 02111 USA

[2] TUFTS UNIV, SCH MED, BOSTON, MA USA

[3] BIOL MARINE LAB, PHYSIOL CELLULAR & MOLEC BIOL COURSE 1994, WOODS HOLE, MA USA

来源：

FEBS LETTERS | 1995年 / 363卷 / 1-2期

关键词：

G-PROTEIN; GUANOSINE TRIPHOSPHATE; CELL SURFACE RECEPTOR; BLOOD PLATELET; SIGNAL TRANSDUCTION; 2ND-MESSENGER SYSTEM;

D O I：

10.1016/0014-5793(95)00278-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thrombin receptor is a G protein-coupled receptor, but the G proteins functionally coupled to this receptor in human platelets are not yet definitively identified. Thrombin stimulation of platelets leads to phospholipase C-mediated increases in intracellular calcium, and previous studies have suggested that the thrombin receptor is coupled to members of the G(q) family. We now demonstrate direct GTPase activation by thrombin receptor activation peptide (TRAP) in human platelet membranes, and specific inhibition of TRAP-activated GTPase by antibodies to G(q). These data demonstrate functional coupling of the thrombin receptor to a member of the G(q) family.

引用

页码：49 / 52

页数：4

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