PROTEIN-KINASE-C INHIBITORS INDUCE APOPTOSIS IN HUMAN-MALIGNANT GLIOMA CELL-LINES

被引：194
|
作者：
COULDWELL, WT
HINTON, DR
HE, SK
CHEN, TC
SEBAT, I
WEISS, MH
LAW, RE
机构：
[1] UNIV SO CALIF,SCH MED,DEPT PATHOL,LOS ANGELES,CA 90033
[2] UNIV SO CALIF,SCH MED,DEPT MED,DIV ENDOCRINOL DIABET & HYPERTENS,LOS ANGELES,CA 90033
来源：
FEBS LETTERS | 1994年 / 345卷 / 01期
关键词：
PROTEIN KINASE C; APOPTOSIS; GLIOMA; STAUROSPORINE; TAMOXIFEN;
D O I：
10.1016/0014-5793(94)00415-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Previous work has demonstrated the importance of the protein kinase C (PKC) system in regulating glioma growth, and has led to clinical trials utilizing PKC inhibitors as adjuncts in the therapy of patients harboring malignant gliomas. This study was performed to explore the possibility that inhibition of PKC in gliomas was triggering an apoptosis signal. Glioma cell lines were treated with PKC inhibitors staurosporine (10 nM), and tamoxifen (10 mu M). DNA from cells treated with each of these drugs exhibited a 'ladder' pattern of oligonucleosome-sized fragments characteristic of apoptosis, thus suggesting that in glioma cells, these drugs may be cytocidal in action.
引用
收藏
页码：43 / 46
页数：4
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