CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING RHIZOPUS-NIVEUS LIPASE

被引：31
|
作者：
KUGIMIYA, W
OTANI, Y
KOHNO, M
HASHIMOTO, Y
机构：
[1] Central Research Institute, Fuji Oil Co., Ltd., Ibaraki 300-24, 4-3 Kinunodai, Yawara, Tsukuba-gun
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 05期
关键词：
D O I：
10.1271/bbb.56.716
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.
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页码：716 / 719
页数：4
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