Effect of Carbon Source and Lipase Inducers on Extracellular Lipase Production by Bacillus sp BK-L40

The bacteria isolated from soil and screened for lipase by spirit blue agar medium. Among the isolated bacterial strains, one strain (BK-L40) was exhibited higher clear zone (higher lipase activity). Further, the selected strain was identified as Bacillus sp. based on the morphological and physiological characteristics and the 16S rRNA sequencing. The highest production of enzyme was obtained in medium supplemented with 1% lactose as carbon source in LB broth on 48 h of incubation. The lipase activity was decreased in all the tested lipase inducers, but the activity was completely nil in the case of Tween 80. This result confirmed that the carbon source is able to stimulate and the lipid source is repressed the synthesis of lipase.