THE PRESENCE OF 2 CLASSES OF HIGH-AFFINITY CYCLOSPORINE-A BINDING-SITES IN MITOCHONDRIA - EVIDENCE THAT THE MINOR COMPONENT IS INVOLVED IN THE OPENING OF AN INNER-MEMBRANE CA2+-DEPENDENT PORE

The inner membrane of rat liver mitochondria contains a reversible Ca2+-dependent pore, opening of which is largely blocked by cyclosporin A. Analyses of [H-3]cyclosporin binding to rat liver mitochondria demonstrate two classes of high-affinity binding site with capacities of < 5 pmol and approximately 60 pmol cyclosporin .mg mitochondrial protein-1 in addition to partitioning into membrane phospholipids (0.03 pmol .mg mitochondrial protein .nM-1). Direct measurement [C-14]sucrose entry into the matrix space indicates that cyclosporin A inhibits pore opening by interacting with the low-capacity sites. The same low-capacity sites (K(d) cyclosporin, 8 nM) are possibly attributable to peptidylprolyl cis-trans-isomerase, although investigation of pore state interconversion from the rapid kinetics of [C-14]sucrose entrapment in the matrix space does not indicate that cyclosporin-sensitive prolyl isomerization occurs at the actual step of pore opening/closure. It is suggested that the low-capacity cyclosporin-binding component may stabilize the open pore state; this is supported by the observations that Ca2+ decreases cyclosporin binding to this component and that cyclosporin brings about closure of the pre-opened pore. The implications for the possible number of functional pores in mitochopdria are discussed.