DISPERSION IN PACKED-COLUMN HYDRODYNAMIC CHROMATOGRAPHY

被引：36
|
作者：
STEGEMAN, G [1 ]
KRAAK, JC [1 ]
POPPE, H [1 ]
机构：
[1] UNIV AMSTERDAM,ANALYT CHEM LAB,NIEUWE ACHTERGRACHT 166,1018 WV AMSTERDAM,NETHERLANDS
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 634卷 / 02期
关键词：
D O I：
10.1016/0021-9673(93)83001-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Zone broadening of dissolved polystyrenes in packed-column hydrodynamic chromatography (HDC) was studied for a column filled with 1.5-mum monodisperse non-porous particles. The reduced plate heights were observed to be almost constant with mobile phase velocity for reduced mobile phase velocities in a range 2->150. In this velocity range, reduced plate heights well below 2 were obtained for polymer samples with molecular masses of 2200, 43 900 and 775 000. A comparison was made between measured plate heights and chromatographic theories of zone spreading. The effects of polymer size, polydispersity and polymer hydrodynamics on the measured plate heights are discussed.
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页码：149 / 159
页数：11
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