RAT (AND MOUSE) MONOCLONAL-ANTIBODIES .8. ELISA MEASUREMENT OF IG PRODUCTION IN MOUSE HYBRIDOMA CULTURE SUPERNATANTS

We describe a rat monoclonal antibody (MAb)-based solid-phase immunoenzymometric assay for the quantification of mouse hybridoma culture supernatant immunoglobulins (Igs). This assay involves the use of two rat MAbs, LO-MK-1 and LO-MK-2, which bind distinct mouse κ light chain epitopes expressed by all murine κ Igs. The assay permits reliable measurement of all murine κ IgG subclasses in the 2-120 ng/ml range and murine κ IgM class in the 2-30 ng/ml range. The intra- and interassay coefficients of variation in the measurement of Ig in mouse hybridoma culture supernatants averaged 7.5% and 5% respectively. The assay is simple, reproducible, rapid and does not require specific equipment. It is of potential value to all laboratories engaged in hybridoma technology. © 1990.