GENERATION OF TRANSGENIC DAIRY-CATTLE USING INVITRO EMBRYO PRODUCTION

被引：0
|
作者：
KRIMPENFORT, P
RADEMAKERS, A
EYESTONE, W
VANDERSCHANS, A
VANDENBROEK, S
KOOIMAN, P
KOOTWIJK, E
PLATENBURG, G
PIEPER, F
STRIJKER, R
DEBOER, H
机构：
[1] GENE PHARMING EUROPE BV, DEPT EMBRYOL, NIELS BOHRWEG 11-13, 2333 CA LEIDEN, NETHERLANDS
[2] RES INST ANIM PROD SCHOONOORD, 3700 AM ZEIST, NETHERLANDS
[3] GENE PHARMING EUROPE BV, DEPT MOLEC BIOL, 2333 CA LEIDEN, NETHERLANDS
[4] LEIDEN UNIV, DEPT BIOCHEM, 2300 RA LEIDEN, NETHERLANDS
来源：
BIO-TECHNOLOGY | 1991年 / 9卷 / 09期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.
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页码：844 / 847
页数：4
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