ISOLATION OF 3 ALLERGENIC FRACTIONS OF THE MAJOR ALLERGEN FROM OLEA-EUROPEA POLLEN AND N-TERMINAL AMINO-ACID-SEQUENCE

被引：54
|
作者：
VILLALBA, M
LOPEZOTIN, C
MARTINOROZCO, E
MONSALVE, RI
PALOMINO, P
LAHOZ, C
RODRIGUEZ, R
机构：
[1] FDN JIMENEZ DIAZ,DEPT INMUNOL,MADRID,SPAIN
[2] UNIV OVIEDO,FAC MED,DEPT BIOL FUNC,OVIEDO,SPAIN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90704-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method to isolate the major allergen from olive pollen (Ole e I) in high yeild is described. The allergenic fraction has been separated into 3 subfractions by reversephase HPLC. All these fractions were reactive to allergic sera from olive-sensitized patients, giving similar responses. No significant differences were observed between the amino acid compositions of these three proteins. The amino acid sequence of the first 27 amino acid residues from the N-terminal end is given. No homologies have been detected between Ole e I and other known allergens obtained from pollen. © 1990.
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页码：523 / 528
页数：6
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