Effects of mesenchymal stromal cells on monocyte differentiation to M1 phenotype and M1/M2 macrophage switching

被引:0
|
作者
Shevela, E. Ya. [1 ]
Sakhno, L. V. [1 ]
Tikhonova, M. A. [1 ]
Batorov, E. V. [1 ]
Ostanin, A. A. [1 ]
Chernykh, E. R. [1 ]
机构
[1] RIFCI, Fed State Budgetary Sci Inst, 14 Yadrintsevskaya Str, Novosibirsk 630099, Russia
来源
BYULLETEN SIBIRSKOY MEDITSINY | 2018年 / 17卷 / 01期
关键词
Mesenchymal stromal cells; monocytes; macrophages; M1 -> M2 polarization; cytokines; chemokines;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. Mesenchymal stromal cells (MSCs) promote the differentiation of unprimed macrophages (Mp) or classically activated M1 cells towards alternatively activated, M2 macrophages. The aim of the work was to study the ability of MSC to induce M1 -> M2 switching by comparing the MSC effects on polarized M1 macrophages and monocytes stimulated by granulocyte-macrophage colony-stimulating factor to M1 differentiation. Material and methods. MSC were co-cultured with monocytes for 7 days or M1 macrophages for 48 hours in Transwell system to prevent direct cell-to-cell contacts. To characterize generated Mp, classical M2 marker CD206, allostimulatory activity in a mixed lymphocyte culture (MLC), and the ability to secrete pro-/anti-inflammatory mediators were analyzed. Results. Co-cultivation of MSCs and M1 macrophages led to the appearance of phenotypic (increased expression of CD206) and functional (decrease in allostimulatory activity) features of M2 phenotype. When MSCs were cultured with monocytes in the M1-inducing medium, generated M. elicited a pronounced stimulating activity in MLC similar to that of M1 (stimulation index 3.45 and 3.4, p = 0.46) and significantly higher than allostimulatory activity of M2 cells (3.45 vs 2.2, p = 0.03). In addition, MSCs did not influence the expression of CD206, as well as the production of pro-(IL-1 beta, TNF-alpha, IL-6, IL-12) and anti-inflammatory (IL1-ra, IL-4, IL-10) cytokines, immunoregulatory cytokines (IFN-gamma, IL-17) and chemokines (IP-10, MCP-1, MIP-1b, Rantes, Eotaxin). Conclusions: The ability of MSC to induce the M2 phenotype depends on the stage of differentiation of monocyte/macrophages. MSCs promote M1 -> M2 switching in cultures of polarized M1 macrophages. In contrast, when MSCs interacted with monocytes in M1-inducing medium, a population of M1-like macrophages is formed with high allostimulatory activity and typical for M1 spectrum of produced cytokines and chemokines.
引用
收藏
页码:167 / 176
页数:10
相关论文
共 50 条
  • [1] miRNA modulation of M1/M2 macrophage phenotype
    Guerau-de-Arellano, Mireia
    Jablonski, Kyle
    Amici, Stephanie
    Gaodet, Andrew
    Popovich, Philip
    [J]. JOURNAL OF IMMUNOLOGY, 2014, 192
  • [2] Macrophage M1/M2 polarization
    Chen Yunna
    Hu Mengru
    Wang Lei
    Chen Weidong
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2020, 877
  • [3] M1/M2 SKIN MACROPHAGE PHENOTYPE IS ALTERED IN DIABETES
    Tellechea, A.
    Leal, E. C.
    Tecilazich, F.
    Auster, M.
    Nabzdyk, L. Pradhan
    Veves, A.
    [J]. WOUND REPAIR AND REGENERATION, 2013, 21 (02) : A45 - A45
  • [4] The effects of M1 macrophage activation on M1-to-M2 phenotypic switching
    O'Brien, Erin M.
    Spiller, Kara L.
    [J]. WOUND REPAIR AND REGENERATION, 2020, 28 : S19 - S20
  • [5] DIFFERENT ROLES OF M1/M2 MACROPHAGE PHENOTYPE FOR RENAL CRYSTAL FORMATION
    Taguchi, Kazumi
    Okada, Atsushi
    Unno, Rei
    Fujii, Yasuhiro
    Hirose, Yasuhiko
    Usami, Masayuki
    Hamamoto, Shuzo
    Ando, Ryosuke
    Itoh, Yasunori
    Tozawa, Keiichi
    Kohri, Kenjiro
    Yasui, Takahiro
    [J]. JOURNAL OF UROLOGY, 2016, 195 (04): : E886 - E887
  • [6] Selenoprotein induced M1 to M2 murine macrophage phenotype switching is imperative in helminth parasite clearance
    Nelson, Shakira M.
    Carlson, Bradley
    Urban, Joseph
    Lei, Xingen
    Hatfield, Dolph
    Prabhu, K. Sandeep
    [J]. FASEB JOURNAL, 2012, 26
  • [7] Enhanced Activity of the Macrophage M1/M2 Phenotypes and Phenotypic Switch to M1 in Periodontal Infection
    Yu, Ting
    Zhao, Li
    Huang, Xin
    Ma, Chanjuan
    Wang, Yixiong
    Zhang, Jincai
    Xuan, Dongying
    [J]. JOURNAL OF PERIODONTOLOGY, 2016, 87 (09) : 1092 - 1102
  • [8] M1和M2
    [J]. 数据, 2004, (06) : 49 - 49
  • [9] DIFFERENTIAL RESPONSES OF M1 AND M2 MONOCYTE-DERIVED MACROPHAGE PHENOTYPES IN COPD
    Ward, A. J. N.
    Chana, K. K.
    Thomas, C. M.
    Fenwick, P. S.
    Donnelly, L. E.
    [J]. THORAX, 2011, 66 : A116 - A116
  • [10] Umbelliprenin Increases the M1/M2 Ratio of Macrophage Polarization and Improves the M1 Macrophage Activity in THP-1 Cells Cocultured with AGS Cells
    MohammadTaher, Bahrami
    Haji Molla Hoseini, Mostafa
    Rezaei, Mitra
    Ziai, Seyed Ali
    [J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2021, 2021