RESEARCH REGARDING THE IDENTIFICATION OF GLOBODERA SPP. USING MORPHOLOGICAL CHARACTERS AND POLYMERASE CHAIN REACTION IN ROMANIA

The control of potato cyst nematodes Globodera rostochiensis (Wollenweber) Behrens and Globodera pallida (Stone) is regulated by Directive 2007/33 EC, which is transposed in Romanian legislation by 139/2010 Ministerial Order. These species are included in the list of harmful organisms of potato which are monitored annually. Both species were detected in the soil samples from Brasov, Covasna, Harghita County in 2010-2011 period. The detection of potato cyst nematodes from soil was carried out with Schuiling centrifuge. The identification of potato cyst nematodes species was based on a combination of morphological, morphometric characters and molecular technique (Multiplex-PCR). It is presented our results regarding criteria, materials and methods which were used for identification of these dangerous pest allover the world and in EU states. In order to investigate species and distribution of the Potato Cyst Nematode (PCN), Globodera spp., present in Romania, soil samples were collected from different fields. Identification of Globodera species was based on some morphological criteria and confirmed by PCR. This is the first report of molecular identification of PCN populations which was done in Romania.