THE CARDIAC CONDUCTION SYSTEM IN THE RAT EXPRESSES THE ALPHA-2 AND ALPHA-3 ISOFORMS OF THE NA+,K+-ATPASE

The sodium pump is crucial for the function of the heart and of the cardiac conduction system, which initiates the heartbeat. The alpha (catalytic) subunit of this pump has three isoforms; the alpha-1 isoform is ubiquitous, but the alpha-2 and alpha-3 isoforms are localized to excitable tissue. Because rodent alpha-2 and alpha-3 isoforms are relatively sensitive to ouabain, which also slows cardiac conduction, we studied heart-cell-specific expression of pump isoform genes. Multiple conduction-system structures, including sinoatrial node, bundle branches, and Purkinje strands, had prominent, specific hybridization signal for alpha-2 and alpha-3 isoforms compared with adjacent working myocytes. This gene-expression approach may be useful for labeling conduction tissue and also for localizing specific membrane channels and receptors in this system.