ISOLATION AND CHARACTERIZATION OF A PROTEASE FROM THE MARINE SPONGE SPHECIOSPONGIA-VESPARIA

被引：9
|
作者：
ARREGUIN, R [1 ]
ARREGUIN, B [1 ]
SORIANOGARCIA, M [1 ]
HERNANDEZARANA, A [1 ]
RODRIGUEZROMERO, A [1 ]
机构：
[1] UNIV AUTONOMA METROPOLITANA IZTAPALAPA, DEPT QUIM, IZTAPALAPA 09340, DF, MEXICO
来源：
FEBS LETTERS | 1993年 / 320卷 / 03期
关键词：
METALLOPROTEASE; SPONGE; SPHECIOSPONGIA-VESPARIA;
D O I：
10.1016/0014-5793(93)80593-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A protein that showed activity against proteic (casein and hide powder azure) and synthetic (BAEE and HLPA) substrates was isolated from the marine sponge Spheciospongia vesparia. The protease was purified from an aqueous extract by ammonium sulfate precipitation, gel filtration, hydrophobic and HPLC-anion exchange chromatographies. The purified protease showed a single band in SDS-PAGE minigels and had a molecular weight of 29,600, but when submitted to isoelectric focusing it showed 2 bands with isoelectric points of 4.56 and 4.43. Its catalytic action was inhibited by EDTA and 1,10-phenanthroline, so it seemed to be a metalloprotease.
引用
收藏
页码：235 / 238
页数：4
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