DEVELOPMENT OF A SOLID-PHASE ENZYME-IMMUNOASSAY FOR THE DETECTION OF ANTI-RO AUTOANTIBODIES

被引：0
|
作者：
DORNER, T
HIEPE, F
MIELKE, F
KIESSIG, ST
LUKOWSKY, A
APOSTOLOFF, E
机构：
[1] HUMBOLDT UNIV,MED SCH CHARITE,DEPT INTERNAL MED,SCHUMANNSTR 20-21,O-1040 BERLIN,GERMANY
[2] HUMBOLDT UNIV,MED SCH CHARITE,INST MED IMMUNOL,O-1086 BERLIN,GERMANY
来源：
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY | 1991年 / 20卷 / 04期
关键词：
ANTI-RO-AUTOANTIBODIES; SYSTEMIC LUPUS ERYTHEMATOSUS; ENZYME IMMUNOASSAY; RO-ANTIGEN PURIFICATION; FAST PROTEIN LIQUID CHROMATOGRAPHY;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter immunoelectrophoresis (r(s) = 0,893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.
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页码：267 / 273
页数：7
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