VASOACTIVE INTESTINAL POLYPEPTIDE STIMULATES CYCLIC-AMP PRODUCTION IN MOUSE N1E-115 NEUROBLASTOMA-CELLS - MODULATION BY A PROTEIN-KINASE-C ACTIVATOR AND IONOMYCIN

被引：6
|
作者：
INUKAI, T
CHIK, CL
HO, AK
机构：
[1] UNIV ALBERTA,FAC MED,DEPT PHYSIOL,EDMONTON T6G 2H7,AB,CANADA
[2] UNIV ALBERTA,FAC MED,DEPT MED,EDMONTON T6G 2H7,AB,CANADA
来源：
PEPTIDES | 1994年 / 15卷 / 08期
基金：
英国医学研究理事会;
关键词：
CAMP; NEUROBLASTOMA CELLS; PROTEIN KINASE C; IONOMYCIN; VIP;
D O I：
10.1016/0196-9781(94)90109-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we investigated the vasoactive intestinal polypeptide (VIP)-stimulated cAMP production and its interaction with protein kinase C activation and elevation of intracellular Ca2+ in N1E-115 neuroblastoma cells. VIP treatment caused a 55-fold increase in cAMP accumulation. Addition of 4 beta-phorbol 12-myristate 13-acetate reduced VIP- but not forskolin-stimulated cAMP response. In comparison, ionomycin potentiated both VIF- and forskolin-induced cAMP accumulation. Our results indicate that VIP stimulates cAMP accumulation in N1E-115 cells, and that although activation of protein kinase C inhibits the VIP-stimulated cAMP response, elevation of intracellular Ca2+ potentiates this signaling pathway.
引用
收藏
页码：1361 / 1365
页数：5
相关论文
共 50 条
  • [1] DISSOCIATION OF PHOSPHORYLATION AND TRANSLOCATION OF A MYRISTOYLATED PROTEIN-KINASE-C SUBSTRATE (MARCKS PROTEIN) IN C6 GLIOMA AND N1E-115 NEUROBLASTOMA-CELLS
    BYERS, DM
    PALMER, FBSC
    SPENCE, MW
    COOK, HW
    [J]. JOURNAL OF NEUROCHEMISTRY, 1993, 60 (04) : 1414 - 1421
  • [2] STUDY OF THE MUSCARINIC RECEPTOR SUBTYPES IN N1E-115 MOUSE NEUROBLASTOMA-CELLS
    FRAEYMAN, NH
    BUYSE, MAM
    LEFEBVRE, RA
    [J]. PHARMACOLOGICAL RESEARCH, 1991, 23 (01) : 33 - 40
  • [3] PRODUCTION OF AN EDRF-LIKE ACTIVITY IN THE CYTOSOL OF N1E-115 NEUROBLASTOMA-CELLS
    GORSKY, LD
    FORSTERMANN, U
    ISHII, K
    MURAD, F
    [J]. FASEB JOURNAL, 1990, 4 (05): : 1494 - 1500
  • [4] THAPSIGARGIN ACTIVATES CALCIUM CURRENT, YET BLOCKS CYCLIC-GMP PRODUCTION IN N1E-115 NEUROBLASTOMA-CELLS
    MATHES, C
    ALOUSI, AA
    THOMPSON, SH
    [J]. BIOPHYSICAL JOURNAL, 1994, 66 (02) : A238 - A238
  • [5] CALCIUM REQUIREMENT FOR CGMP PRODUCTION DURING MUSCARINIC ACTIVATION OF N1E-115 NEUROBLASTOMA-CELLS
    THOMPSON, SH
    MATHES, C
    ALOUSI, AA
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1995, 269 (04): : C979 - C985
  • [6] MODULATION OF CYCLIC-AMP METABOLISM BY PROTEIN-KINASE-C IN PC18 CELLS
    YINGLING, JD
    FULLER, LZ
    JACKSON, BA
    [J]. NEUROSCIENCE LETTERS, 1994, 166 (02) : 157 - 160
  • [7] DESENSITIZATION OF MUSCARINIC M1-RECEPTORS OF MURINE NEUROBLASTOMA-CELLS (CLONE N1E-115) WITHOUT RECEPTOR DOWN-REGULATION AND PROTEIN-KINASE-C ACTIVITY
    KANBA, S
    KANBA, KS
    MCKINNEY, M
    PFENNING, M
    ABRAHAM, R
    NOMURA, S
    ENLOES, L
    MACKEY, S
    RICHELSON, E
    [J]. BIOCHEMICAL PHARMACOLOGY, 1990, 40 (05) : 1005 - 1014
  • [8] THAPSIGARGIN SELECTIVELY STIMULATES SYNTHESIS OF PHOSPHATIDYLGLYCEROL IN N1E-115 NEUROBLASTOMA-CELLS AND PHOSPHATIDYLINOSITOL IN C6 GLIOMA-CELLS
    PALMER, FBS
    COOK, HW
    BYERS, DM
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1215 (1-2): : 190 - 197
  • [9] DIRECT MODULATION OF NA+ CURRENTS BY PROTEIN-KINASE-C ACTIVATORS IN MOUSE NEUROBLASTOMA-CELLS
    RENGANATHAN, M
    GODOY, CMG
    CUKIERMAN, S
    [J]. JOURNAL OF MEMBRANE BIOLOGY, 1995, 144 (01): : 59 - 69
  • [10] ACTIVATION OF PROTEIN-KINASE-C POTENTIATES CYCLIC-AMP PRODUCTION AND STIMULATES STEROIDOGENESIS IN DIFFERENTIATED OVARIAN GRANULOSA-CELLS
    SHINOHARA, O
    KNECHT, M
    FENG, P
    CATT, KJ
    [J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1986, 24 (01): : 161 - 168
12345