NTRBC-DEPENDENT EXPRESSION FROM THE RHIZOBIUM-MELILOTI DCTA PROMOTER IN ESCHERICHIA-COLI

被引：0
|
作者：
ALLAWAY, D [1 ]
BOESTEN, B [1 ]
OGARA, F [1 ]
机构：
[1] NATL UNIV IRELAND UNIV COLL CORK,DEPT MICROBIOL,CORK,IRELAND
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 128卷 / 03期
关键词：
2 COMPONENT SYSTEMS; CROSS-TALK; SIGMA(54)-REGULATORS; NTRBC; RHIZOBIUM MELILOTI DCTA; ESCHERICHIA COLI; DCTBD;
D O I：
10.1016/0378-1097(95)00099-Q
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulatory levels and under different physiological conditions in the heterologous host. However, NtrBC-dependent cross-talk was not observed in free-living R. meliloti under the conditions tested. Comparisons of the predicted amino acid sequences of DctD and NtrC from various sources indicated a specific region of the NtrC from rhizobia, which may have diverged from a consensus NtrC/DctD sequence to minimise interference between the two component systems, NtrBC and DctBD.
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页码：241 / 245
页数：5
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