Simultaneous determination of seven compounds in Lonicera japonica by high performance liquid chromatography

A high performance liquid chromatographic HPLC method was developed to determine the contents of seven compounds in Lonicera japonica and Folium Lonicerae with maximum wavelength conversion program and analyze the content differences between them. The separation was performed on an Agilent Eclipse Plus C18 column 250 mm x 4.6 mm 5 mu m operated at normal temperature with the gradient elution by two mobile phases of water including 0. 3% v/v formic acid A and acetonitrile B at a flow rate of 1 mL/min. The maximum detection wavelength was set at 330 nm and 350 nm by conversion. The contents of chlorogenic acid and caffeic acid in Lonicera new leaves were 2. 572% and 1.498%o respectively both higher than those cited in Chinese Pharmacopoeia indicating that Lonicera new leaves have the necessity to be further studied and developed. This method is simple rapid and highly sensitive. It is suitable for the simultaneous determination of the seven compounds and the quality control of Lonicera japonica.