INTERFERENCE OF MG2+ AND CA2+ ON PROTEIN DETERMINATION WITH LOWRYS METHOD

被引：5
|
作者：
XIE, QS
BURNELL, GM
机构：
[1] Department of Zoology, University College Cork
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 107卷 / 04期
关键词：
INTERFERENCE OF MG2+; LOWRYS METHOD;
D O I：
10.1016/0305-0491(94)90192-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of Mg2+ (0.84-6.74 mM) and Ca2+ (0.18-1.45 mM) on the Lowry method was studied. Mg2+ and Ca2+ affected the absorbance of both the protein-free blank and protein samples. The tolerable limit was <0.84 mM for Mg2+ and 0.55 mM for Ca2+. The interference by Mg2+ was more significant than Ca2+ at the same concentration. A hypothesis was proposed in respect of the mechanism of interference by Mg2+: the Mg(OH)(2) precipitate binds with protein and some constituent of Lowry reagent B and thus reduces their sensitivity to Folin reagent.
引用
收藏
页码：605 / 608
页数：4
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