AUTONOMOUSLY REPLICATING FRAGMENTS OF MITOCHONDRIAL-DNA FROM PENICILLIUM-URTICAE IN SACCHAROMYCES-CEREVISIAE

被引：2

作者：

YAMAMOTO, H ^{[1
]}

YOSHIDA, Y ^{[1
]}

KITAMURA, Y ^{[1
]}

SEKIGUCHI, J ^{[1
]}

机构：

[1] SHINSHU UNIV,FAC TEXT SCI & TECHNOL,DEPT APPL BIOL,3-15-1 TOKIDA,UEDA,NAGANO 386,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 71卷 / 04期

关键词：

D O I：

10.1016/0922-338X(91)90281-K

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eight fragments which cover the whole range of the mitochondrial genome of Penicillium urticae were subcloned into the yeast integration vector YIp5. Transformation of Saccharomyces cerevisiae with the constructed plasmids by the alkali cation method indicated that six plasmids are able to replicate in yeast. Both closed and open circular forms of the plasmids were detected in the DNA extracts from transformants. Distribution of the autonomously replicating sequence in the mitochondrial genome was similar to that in P. chrysogenum except for one small region.

引用

页码：275 / 277

页数：3

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