The differences in tolerance, morphology, and physiological response of diclofop-resistant and -susceptible wild oat biotypes collected from fields in Saskatchewan, Canada, were investigated under growth room and field conditions. Under herbicide-free conditions the resistant biotype had more upright leaves with about 12% less leaf area and 50% less leaf width than the susceptible biotype. A marked difference in the level of tolerance to diclofop was observed. Photosynthesis was initially significantly reduced in both biotypes after treatment with diclofop at the rate of 0.70 kg ai ha-1, but the resistant biotype was able to recover. Injury to the susceptible biotype was reduced by coating the seeds with 1,8-naphthalic anhydride. Differential foliar retention was not an important factor in selectivity of diclofop among the two biotypes.