MAPPING OF THE RNA-BINDING DOMAIN OF THE ALFALFA MOSAIC-VIRUS MOVEMENT PROTEIN

被引：32

作者：

SCHOUMACHER, F ^{[1
]}

GIOVANE, C ^{[1
]}

MAIRA, M ^{[1
]}

POIRSON, A ^{[1
]}

GODEFROYCOLBURN, T ^{[1
]}

BERNA, A ^{[1
]}

机构：

[1] UNIV STRASBOURG 1, INST BIOL MOLEC PLANTES, F-67084 STRASBOURG, FRANCE

来源：

JOURNAL OF GENERAL VIROLOGY | 1994年 / 75卷

关键词：

D O I：

10.1099/0022-1317-75-11-3199

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In-frame contiguous deletions were created in the movement protein gene of alfalfa mosaic virus by site-directed mutagenesis. The mutated movement proteins were expressed in Escherichia coli, extracted and then purified by denaturing gel electrophoresis and then renatured. Their binding ability with RNA was assayed by electrophoretic retardation and u.v.-crosslinking. Results indicated that a domain included within amino acids 36 to 81 was necessary for RNA binding.

引用

页码：3199 / 3202

页数：4

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