PROTECTIVE IMMUNITY IN BABOONS VACCINATED WITH A RECOMBINANT ANTIGEN OR RADIATION-ATTENUATED CERCARIAE OF SCHISTOSOMA-MANSONI IS ANTIBODY-DEPENDENT

Mice vaccinated with radiation-attenuated cercariae of Schistosoma mansoni exhibit high levels of resistance to challenge infection. We have previously shown that sera from these mice recognize polypeptides that are expressed on the surface of newly transformed schistosomula. We have cloned and sequenced a cDNA that encodes a 62-kDa portion of one of these polypeptides. Vaccination of mice with this 62-kDa polypeptide (designated rIrV-5) elicits high antibody titers and significant resistance to challenge infection. We report here the results of a vaccination trial in baboons with the rIrV-5 or radiation-attenuated cercariae. rIrV-5 was presented either in the form of protein micelles or complexed with the outer membrane protein of meningococcus to form proteosomes. The level of protection achieved in these groups ranged from 0 to 54%, with a mean of 27.7%. In baboons exposed to radiation-attenuated cercariae the level of protection was very high, with a mean of 84%. The resistance observed after vaccination with rIrV-5 or radiation-attenuated cercariae was reflected in the overall histopathology. Vaccination of baboons with rIrV-5 or radiation-attenuated cercariae elicited an antibody response against epitopes exposed on the surface of newly transformed schistosomula. in the case of baboons vaccinated with radiation-attenuated cercariae, this response was not limited to epitopes encompassed by rIrV-5. Analysis of individual baboon sera by ELISA demonstrated that there was a direct correlation between the anti-rIrV-5 titer and resistance to challenge worm burden, suggesting that the immunoprotective mechanism is antibody-dependent.