ENUMERATION OF VIABLE CANDIDA-ALBICANS BLASTOSPORES USING TETRABROMOFLUORESCEIN (EOSIN-Y) AND FLOW-CYTOMETRY

被引：9
|
作者：
COSTANTINO, PJ
BUDD, DE
GARE, NF
机构：
[1] School of Biomedical Sciences, Curtin University of Technology, Perth, Western Australia, Kent Street, Bentley
来源：
CYTOMETRY | 1995年 / 19卷 / 04期
关键词：
COLONY FORMING UNITS; VIABILITY TESTING; SYSTEMIC CANDIDIASIS; CELL RESPIRATION;
D O I：
10.1002/cyto.990190413
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid assay was developed for determination of the viability of blastospores of Candida albicans utilizing now cytometry to detect the accumulation of tetrabromofluorescein in non-viable yeast cells. Eosin Y was shown to stain non-viable C. albicans blastospores selectively without affecting the cellular viability of competent yeast cells or resulting in non-specific staining of viable cells. Results of these studies show that this now cytometric method of determining cell viability of C. albicans is more accurate and more precise than the more common method of enumerating colony forming units. (C) 1995 Wiley-Liss, Inc.
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页码：370 / 375
页数：6
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