NEW APPROACH TO WATER-SOLUBLE CARBOHYDRATE DETERMINATION AS A TOOL FOR EVALUATION OF PLANT-CELL WALL DEGRADING ENZYMES

被引：53
|
作者：
SLOMINSKI, BA
GUENTER, W
CAMPBELL, LD
机构：
[1] Department of Animal Science, University of Manitoba, Winnipeg, Manitoba
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 12期
关键词：
D O I：
10.1021/jf00036a016
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Studies were conducted to develop a rapid method for the determination of total water-soluble carbohydrates in feedstuffs. Conditions required to result in the satisfactory hydrolysis of plant carbohydrates using HCl without substantial decomposition of constituent sugars were established. The method was validated by recovery studies utilizing mixtures of pure carbohydrates and by comparison of results with those determined by individual analysis of soluble polysaccharides in canola samples. In addition, the total water-soluble carbohydrate content of a number of different feedstuffs was determined, and the application of the method in the evaluation of the efficacy of various commercially available carbohydrase-like enzyme preparations on the hydrolysis of canola meal cell wall polysaccharides was demonstrated.
引用
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页码：2304 / 2308
页数：5
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