DETECTION OF THE BOVINE LEUKEMIA-VIRUS BY THE POLYMERASE CHAIN-REACTION

被引：0
|
作者：
KUZMAK, J [1 ]
MOUSSA, A [1 ]
GRUNDBOECKJUSKO, J [1 ]
机构：
[1] CNEVA LAB,DEPT VIROL,F-69342 LYONS,FRANCE
来源：
ACTA BIOCHIMICA POLONICA | 1991年 / 38卷 / 01期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The polymerase - chain reaction was applied for detection of provirus DNA of the bovine leukaemia virus (BLV). A short fragment of 292 bp including region R and U5 LTR 5' of BLV was amplified, and the optimum parameters of amplification of this fragment were established. Electrophoresis revealed the presence of the 292 bp fragment from the leucocytes of four out of six cows showing a positive serological response to BLV antigens. Application of the polymerase-chain reaction in diagnosis of bovine leukaemia is suggested.
引用
收藏
页码：107 / 110
页数：4
相关论文
共 50 条
  • [1] DETECTION OF BOVINE LEUKEMIA-VIRUS IN CATTLE BY THE POLYMERASE CHAIN-REACTION
    MURTAUGH, MP
    LIN, GF
    HAGGARD, DL
    WEBER, AF
    MEISKE, JC
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1991, 33 (1-2) : 73 - 85
  • [2] DETECTION OF BOVINE LEUKEMIA-VIRUS RNA IN SERUM USING THE POLYMERASE CHAIN-REACTION
    POON, H
    JIMENEZ, E
    JACOBS, RM
    SONG, ZL
    JEFFERSON, B
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1993, 41 (01) : 101 - 112
  • [3] EARLY DETECTION OF BOVINE LEUKEMIA-VIRUS IN CATTLE BY USE OF THE POLYMERASE CHAIN-REACTION
    KELLY, EJ
    JACKSON, MK
    MARSOLAIS, G
    MORREY, JD
    CALLAN, RJ
    [J]. AMERICAN JOURNAL OF VETERINARY RESEARCH, 1993, 54 (02) : 205 - 209
  • [4] DIRECT DETECTION OF BOVINE LEUKEMIA-VIRUS INFECTION - PRACTICAL APPLICABILITY OF A DOUBLE POLYMERASE CHAIN-REACTION
    BALLAGIPORDANY, A
    KLINTEVALL, K
    MERZA, M
    KLINGEBORN, B
    BELAK, S
    [J]. JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1992, 39 (01): : 69 - 77
  • [5] USE OF POLYMERASE CHAIN-REACTION TO DIAGNOSE BOVINE LEUKEMIA-VIRUS INFECTION IN CALVES AT BIRTH
    AGRESTI, A
    PONTI, W
    ROCCHI, M
    MENEVERI, R
    MAROZZI, A
    CAVALLERI, D
    PERI, E
    POLI, G
    GINELLI, E
    [J]. AMERICAN JOURNAL OF VETERINARY RESEARCH, 1993, 54 (03) : 373 - 378
  • [6] A FIELD-EVALUATION OF THE POLYMERASE CHAIN-REACTION PROCEDURE FOR THE DETECTION OF BOVINE LEUKEMIA-VIRUS PROVIRAL DNA IN CATTLE
    EAVES, FW
    MOLLOY, JB
    DIMMOCK, CK
    EAVES, LE
    [J]. VETERINARY MICROBIOLOGY, 1994, 39 (3-4) : 313 - 321
  • [7] COMBINED POLYMERASE CHAIN-REACTION HYBRIDIZATION MICROPLATE ASSAY USED TO DETECT BOVINE LEUKEMIA-VIRUS AND SALMONELLA
    RASMUSSEN, SR
    RASMUSSEN, HB
    LARSEN, MR
    HOFFJORGENSEN, R
    CANO, RJ
    [J]. CLINICAL CHEMISTRY, 1994, 40 (02) : 200 - 205
  • [8] RAPID DETECTION OF BOVINE VIRAL DIARRHEA VIRUS BY POLYMERASE CHAIN-REACTION
    LOPEZ, OJ
    OSORIO, FA
    DONIS, RO
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (03) : 578 - 582
  • [9] DETECTION OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS BY THE POLYMERASE CHAIN-REACTION
    NADINDAVIS, SA
    CHANG, SC
    SMITH, H
    JACOBS, RM
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1993, 42 (2-3) : 323 - 336
  • [10] AMPLIFICATION AND ANALYSIS OF SPECIFIC DNA AND RNA SEQUENCES OF BOVINE LEUKEMIA-VIRUS FROM INFECTED COWS BY POLYMERASE CHAIN-REACTION
    SHERMAN, MP
    EHRLICH, GD
    FERRER, JF
    SNINSKY, JJ
    ZANDOMENI, R
    DOCK, NL
    POIESZ, BJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (01) : 185 - 191
12345