INCORPORATION OF EXOGENOUS URACIL BY CRYPTOSPORIDIUM-PARVUM INVITRO

被引：14

作者：

UPTON, SJ ^{[1
]}

TILLEY, M ^{[1
]}

MITSCHLER, RR ^{[1
]}

OPPERT, BS ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI, DEPT BIOCHEM, MANHATTAN, KS 66506 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 05期

关键词：

D O I：

10.1128/JCM.29.5.1062-1065.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Oocysts of Cryptosporidium parvum were used to infect Madin-Darby bovine kidney cells. Cultures were incubated in a reduced-oxygen atmosphere in candle jars or in a 5% CO2-95% air atmosphere. At 72 h, parasites were quantitated microscopically and found to be enhanced 5.5-fold in the reduced-oxygen atmosphere. Using candle jars, we then determined that C. parvum was amenable to [H-3]uracil incorporation assays and easily quantitated with this method.

引用

页码：1062 / 1065

页数：4

共 50 条

[1] INVITRO EXCYSTATION OF CRYPTOSPORIDIUM-PARVUM
ROBERTSON, LJ
CAMPBELL, AT
SMITH, HV
[J]. PARASITOLOGY, 1993, 106 : 13 - 19
[2] INVITRO CULTIVATION OF CRYPTOSPORIDIUM-PARVUM AND SCREENING FOR ANTICRYPTOSPORIDIAL DRUGS
MCDONALD, V
STABLES, R
WARHURST, DC
BARER, MR
BLEWETT, DA
CHAPMAN, HD
CONNOLLY, GM
CHIODINI, PL
MCADAM, KPWJ
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (08) : 1498 - 1500
[3] INVITRO MURINE LYMPHOCYTE BLASTOGENIC RESPONSES TO CRYPTOSPORIDIUM-PARVUM
WHITMIRE, WM
HARP, JA
[J]. JOURNAL OF PARASITOLOGY, 1990, 76 (03) : 450 - 452
[4] STUDIES OF INVITRO EXCYSTATION OF CRYPTOSPORIDIUM-PARVUM FROM CALVES
WOODMANSEE, DB
[J]. JOURNAL OF PROTOZOOLOGY, 1987, 34 (04): : 398 - 402
[5] INVITRO MULTIPLICATION OF CRYPTOSPORIDIUM-PARVUM IN MOUSE PERITONEAL-MACROPHAGES
MARTINEZ, F
MASCARO, C
ROSALES, MJ
DIAZ, J
CIFUENTES, J
OSUNA, A
[J]. VETERINARY PARASITOLOGY, 1992, 42 (1-2) : 27 - 31
[6] INVITRO ACTIVITIES OF LYTIC PEPTIDES AGAINST THE SPOROZOITES OF CRYPTOSPORIDIUM-PARVUM
ARROWOOD, MJ
JAYNES, JM
HEALEY, MC
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (02) : 224 - 227
[7] AN INVITRO MODEL OF ATTACHMENT OF CRYPTOSPORIDIUM-PARVUM SPOROZOITES TO MDCK CELLS
HAMER, DH
WARD, H
TZIPORI, S
PEREIRA, MEA
KEUSCH, GT
[J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, : 48 - 48
[8] STUDIES ON CRYOPRESERVATION OF CRYPTOSPORIDIUM-PARVUM
FAYER, R
NERAD, T
RALL, W
LINDSAY, DS
BLAGBURN, BL
[J]. JOURNAL OF PARASITOLOGY, 1991, 77 (03) : 357 - 361
[9] CRYPTOSPORIDIUM-PARVUM INFECTIONS OF SWINE
LINDSAY, DS
BLAGBURN, BL
[J]. COMPENDIUM ON CONTINUING EDUCATION FOR THE PRACTICING VETERINARIAN, 1991, 13 (05): : 891 - 894
[10] CELLULAR BIOLOGY OF CRYPTOSPORIDIUM-PARVUM
PETERSEN, C
[J]. PARASITOLOGY TODAY, 1993, 9 (03): : 87 - 91

← 1 2 3 4 5 →