A SIMPLE AND ECONOMICAL DRB1 TYPING PROCEDURE COMBINING GROUP-SPECIFIC AMPLIFICATION, DNA HETERODUPLEX AND ENZYME RESTRICTION ANALYSIS

被引：10
|
作者：
DAMATO, M
SORRENTINO, R
机构：
[1] UNIV ROMA LA SAPIENZA,DEPT CELL BIOL & DEV,VIA APULI 1,I-00185 ROME,ITALY
[2] CNR,ROME,ITALY
[3] UNIV LAQUILA,DEPT EXPTL MED,I-67100 LAQUILA,ITALY
来源：
TISSUE ANTIGENS | 1994年 / 43卷 / 05期
关键词：
HLA-DR; GROUP-SPECIFIC PCR; HETERODUPLEX ANALYSIS;
D O I：
10.1111/j.1399-0039.1994.tb02343.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A new procedure for HLA-DRB1 typing is proposed. The method combines six group-specific amplifications with heteroduplex analysis and, in some cases, enzyme restriction analysis. This technique, which is as discriminative as oligotyping, is simple, economical and does not require probes. These characteristics make this approach a valid alternative to other HLA genomic typing procedures, especially in those cases such as donor-recipient pairs matching where a small number of samples has to be managed at once.
引用
收藏
页码：295 / 301
页数：7
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