Complete Nucleotide Sequence of the Mitochondrial DNA from a Liverwort, Marchantia polymorpha

Libraries of cosmid and plasmid clones covering the entire region of mtDNA from the liverwort Marchantia polymorpha were constructed. These clones were used for the determination of the complete nucleotide sequence of the liverwort mtDNA totally 186,608 bp (GenBank no. M68929) and including genes for 3 species of ribosomal RNAs, 29 genes for 27 species of transfer RNAs, and 30 genes for functionally known proteins ( 16 ribosomal proteins, 3 subunits of cytochrome coxidase, apocytochrome b protein, 3 subunits of H*-ATPase, and 7 subunits of NADH ubiquinone oxidoreductase). The genome also contains 32 unidentified open reading frames. Thus the complete nucleotide sequences from both chloroplast and mitochondrial genomes have been determined in the same organism. Plasmid clones are available upon the request.