A KINETOPLAST DNA PROBE DIAGNOSTIC FOR LEISHMANIA-INFANTUM

被引：19
|
作者：
GRAMICCIA, M
SMITH, DF
ANGELICI, MC
READY, PD
GRADONI, L
机构：
[1] NAT HIST MUSEUM,DEPT ENTOMOL,LONDON SW7 5BD,ENGLAND
[2] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AY,ENGLAND
来源：
PARASITOLOGY | 1992年 / 105卷
基金：
英国医学研究理事会;
关键词：
LEISHMANIA-INFANTUM; KINETOPLAST DNA PROBE;
D O I：
10.1017/S0031182000073650
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of L. major, L. tropica, L. killicki, L. aethiopica, L. braziliensis and L. mexicana. A low degree of hybridization was found with L. donovani and L. chagasi. This probe will be of particular use in Mediterranean countries, such as Italy, in which dermotropic zymodemes of L. infantum can be difficult to isolate and grow in numbers sufficient for isoenzyme typing.
引用
收藏
页码：29 / 34
页数：6
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