EXPRESSION OF HUMAN 17-BETA-HYDROXYSTEROID DEHYDROGENASE IN MAMMALIAN-CELLS

被引：72
|
作者：
DUMONT, M
LUUTHE, V
DELAUNOIT, Y
LABRIE, F
机构：
[1] CHU LAVAL,RES CTR,MRC,MOLEC ENDOCRINOL GRP,QUEBEC CITY G1V 4G2,QUEBEC,CANADA
[2] UNIV LAVAL,QUEBEC CITY G1V 4G2,QUEBEC,CANADA
来源：
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1992年 / 41卷 / 3-8期
关键词：
D O I：
10.1016/0960-0760(92)90391-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The insert of 1278 bp containing the entire coding region of cDNA encoding human 17-beta-hydroxysteroid dehydrogenase (17-beta-HSD) was inserted into a pHS1 vector and expressed in HeLA human cervical carcinoma cells and COS-1 monkey kidney tumor cells. Western blot analysis indicated that the expressed protein migrates at the same position as the purified enzyme and is recognized by the antibody raised against purified human placental 17-beta-HSD. The expressed enzyme efficiently catalyzes the interconversion of estrone and estradiol while dehydroepiandrosterone and 5-androstene-3-beta,17-beta-diol are interconverted at a lower rate. The present data suggest the existence of two 17-beta-HSDs.
引用
收藏
页码：605 / 608
页数：4
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