MOUSE IGE RESPONSE AGAINST EXOANTIGENS OF TRYPANOSOMA-CRUZI

This work describes the occurrence of anaphylactic antibodies against exoantigens of Trypanosoma cruzi in infected or immunized mice. The results obtained show that the exoantigens induce an IgE response when injected into mice with complete Freund's adjuvant or in animals infected with 102 trypomasti-gotes. Among the antigens recognized by anti-exoantigen IgE, two molecules of 27 and 45 kD were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by western blotting. Immunofluorescence analysis located some target antigens on the parasite surface. © 1990 S. Karger AG, Basel.