A MAMMALIAN PROTEIN OF 220 KDA BINDS PRE-MESSENGER-RNAS IN THE SPLICEOSOME - A POTENTIAL HOMOLOG OF THE YEAST PRP8 PROTEIN

被引：56
|
作者：
GARCIABLANCO, MA
ANDERSON, GJ
BEGGS, J
SHARP, PA
机构：
[1] MIT,CTR CANC RES,CAMBRIDGE,MA 02139
[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[3] UNIV EDINBURGH,DEPT MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 08期
关键词：
Pre-mRNA splicing; UV-crosslinking;
D O I：
10.1073/pnas.87.8.3082
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.
引用
收藏
页码：3082 / 3086
页数：5
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