CLONING AND MAPPING OF 5' EXONS FROM THE GENE ENCODING CHICKEN BETA-NERVE GROWTH-FACTOR

被引：7
|
作者：
CARRIER, A [1 ]
DEVIGNES, MD [1 ]
ROSIER, MF [1 ]
AUFFRAY, C [1 ]
机构：
[1] CNRS, UPR 420,UNITE GENET MOLEC & BIOL DEV, 7 RUE GUY MOQUET,BP8, F-94801 VILLEJUIF, FRANCE
来源：
GENE | 1992年 / 116卷 / 02期
关键词：
ANCHORED POLYMERASE CHAIN REACTION; RARE MESSENGER; INTERSPECIES SIMILARITY; GENE ORGANIZATION;
D O I：
10.1016/0378-1119(92)90513-O
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
An NGF cDNA containing the 5' exons of the nerve growth factor (NGF) messenger was obtained from chicken heart mRNA using the anchored polymerase chain reaction technique. Alignment of the chicken with the corresponding murine and human sequences reveals interspecies similarities. A sequence corresponding to an exon found only in the NGF messenger, which is abundant in the submaxillary gland of the male mouse, is present in the chicken NGF cDNA. The first non-coding exons of the NGF gene are much less conserved between chicken and mouse or human than the region of the last exon encoding the mature protein. After the cloning of the chicken NGF gene from a cosmid library, the chicken NGF exons have been located within 20 kb of DNA. The chicken NGF gene is therefore shorter than its murine counterpart which spans more than 43 kb. Furthermore, the organization of the chicken and murine NGF genes markedly differs in their 5' portion.
引用
收藏
页码：173 / 179
页数：7
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