PHARMACOLOGICAL CHARACTERIZATION OF [H-3] DOPAMINE AND [H-3] SPIPERONE BINDING IN MOUSE CEREBELLUM

1. [H-3]Dopamine and [H-3]spiperone binding to cerebellar homogenates was characterized utilizing dopaminergic agonists, antagonists and non-dopaminergic drugs. 2. The [H-3]DA binding to low affinity binding sites reveals a heterogenous population consisting of dopaminergic as well as serotonergic and noradrenergic sites. However, the high affinity binding of[H-3]DA reflects dopaminergic sites, although a small contribution of serotonergic and noradrenergic binding sites cannot be excluded. 3. [H-3]Spiperone also labels a heterogenous population of binding sites which, however, are mainly dopaminergic.