CLONING AND SEQUENCE OF THE HUMAN ALPHA-7 NICOTINIC ACETYLCHOLINE-RECEPTOR

被引：31
|
作者：
DOUCETTESTAMM, L
MONTEGGIA, LM
DONNELLYROBERTS, D
WANG, MT
LEE, J
TIAN, JX
GIORDANO, T
机构：
[1] ABBOTT LABS,NEUROSCI RES,DEPT 47W,ABBOTT PK,IL 60064
[2] COLLABORAT RES INC,HUMAN & MOLEC GENET,WALTHAM,MA
来源：
DRUG DEVELOPMENT RESEARCH | 1993年 / 30卷 / 04期
关键词：
NICOTINIC ACETYLCHOLINE RECEPTOR; CLONING; HUMAN; RTPCR;
D O I：
10.1002/ddr.430300408
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A rat clone for the nicotinic acetylcholine receptor (nAChR) alpha 7, generated by reverse transcriptase-polymerase chain reaction (rtPCR) from a region which did not exhibit significant homology to other rat nAChRs, was used to probe human cDNA libraries. A number of clones corresponding to the human alpha 7 were isolated and sequenced. The human sequence showed 87% identity at the nucleic acid level to the rat sequence and 96% identity at the amino acid level. Unlike the rat alpha 7 nAChR, the human gene does not appear to be the most divergent member of the nAChR family. The cloning of the human alpha 7 will make it possible to study the functional formation of human neuronal nAChR channels containing the alpha 7 subtype. (C) 1993 Wiley-Liss, Inc.
引用
收藏
页码：252 / 256
页数：5
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