FLOW-INJECTION STOPPED-FLOW SPECTROFLUOROMETRIC KINETIC DETERMINATION OF TOTAL ASCORBIC-ACID BASED ON AN ENZYME-LINKED COUPLED REACTION

被引：40
|
作者：
HUANG, HP [1 ]
CAI, RX [1 ]
DU, YM [1 ]
ZENG, YN [1 ]
机构：
[1] WUHAN UNIV,DEPT ENVIRONM SCI,WUHAN 430072,PEOPLES R CHINA
来源：
ANALYTICA CHIMICA ACTA | 1995年 / 309卷 / 1-3期
基金：
中国国家自然科学基金;
关键词：
FLOW INJECTION; KINETIC METHODS; FLUOROMETRY; ENZYMATIC METHODS; LACCASE; ASCORBIC ACID;
D O I：
10.1016/0003-2670(95)00056-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow-injection enzymatic kinetic spectrofluorimetric procedure for determining total ascorbic acid in solution by combining the merging zones principle with the stopped-flow technique is described. It is based on the oxidation of ascorbic acid catalysed by laccase to dehydroascorbic acid, which then reacts with o-phenylenediamine to form a fluorescent quinoxaline. Total ascorbic acid is determined in the range of 0.025-1.0 mu g ml(-1) at 30 determinations per h. The procedure is applied to the determination of total ascorbic acid in wine, beer, urine and pharmaceutical preparations.
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页码：271 / 275
页数：5
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