Disruption of Pseudomonas aeruginosa and Staphylococcus aureus biofilm by Manuka and Beri Honey

Aim: Formation and detection of the biofilm in microtiter plate and role of honey in prevention and disruption of this biofilm. Methods: A six months experimental study was carried out at the of Microbiologydepartment university of Health Sciences (UHS) Lahore, Pakistan in which MDR clinical isolate of Staphylococcus aureus and Pseudomonas aeruginosa were used. Biofilm formation and detection was done by microtiter plate method. Two different honeys were used in order to see either they inhibits and disrupt the biofilm or not. Results: Manuka and Beni honey inhibited the biofilm formation of both organism 20% and 30% (w/v) . Both honeys disrupted the established biofilm ofat concentration greater than 40% (w/v). It was clearfrom results that a higherconcentration of both honey was required to inhibit as well as disrupt established biofilm. Conclusions: Staphylococcus aureusand Pseudomonas aeruginosa form strongly adherent biofilm. The conclusion of this study is that honeywhether local or from outside possesses good anti biofilm activity against MDR organism.