Soil phosphorus (P) is an essential nutrient for plant growing, which very often appears as the main limiting element in many agricultural Systems For studying P transformations and identify the effects of soil type and land use on P distribution among the different soil P pools two soil from Villa Clara were selected: brown calcareous soil and Humus-rich calcareous soil, classified as Pardo Mullido Medianamente Lavado y Humico Calcimorfico Tipico Carbonatado, in the Cuban soil classification system (Hernandez et al., 2005) Three land uses were selected for this study: sugarcane, pasture, and woodland For P fractionation was used the methodology from Tiessen and Moir (1993), based on the bioavailability of P pools. The results showed that P distribution and its availability were influenced by management and soil properties. Plant residues and manure increased P levels in pasture and woodland areas. In general, the humus-rich calcareous soil presented higher P content than the brown one. However, in all ecosystems 60% P was retained in non-available forms. The organic P (Po) fraction represented only a 16% in the sugarcane areas, but it was 38% and 36% in the pasture and woodland areas, respectively. Even much lower than the inorganic fraction (Pi), Po constitute an important P source in both soils and it can determine the availability of this nutrient, considering that mist of Pi in non-available for plants.