FORMATION OF CHARGE SEPARATED STATE P(+)Q(A)(-) AND TRIPLET-STATE P-3 AT LOW-TEMPERATURE IN RHODOBACTER-SPHAEROIDES (R-26) REACTION CENTERS IN WHICH BACTERIOPHEOPHYTIN A IS REPLACED BY PLANT PHEOPHYTIN A

Low temperature optical and photochemical properties of Rhodobacter sphaeroides (R-26) reaction centers, in which bacteriopheophytin a has been replaced by plant pheophytin <alpha a, are reported. Modified reaction centers preserve the ability for photoinduced electron transfer from the primary electron donor P to the primary quinone acceptor Q(A) at 80K. The triplet state ESR signal of modified reaction centers with prereduced Q(A) at 10K shows an electron spin polarization pattern and ZFS parameters analogous to those for the triplet state P-3 in non-treated reaction centers. It was found that at low temperature both P(+)Q(A)(-), and P-3 states are formed via a precursor radical pair P+I- in which I is the introduced plant pheophytin molecule. This shows that acceptor systems of bacterial and plant (photosystem II) reaction centers are mutually replacable in structural and functional aspects.