PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR BOVINE GAMMA-INTERFERON

被引：48
|
作者：
WOOD, PR [1 ]
ROTHEL, JS [1 ]
MCWATERS, PGD [1 ]
JONES, SL [1 ]
机构：
[1] COMMONWEALTH SERUM LAB,PARKVILLE,VIC 3052,AUSTRALIA
来源：
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1990年 / 25卷 / 01期
关键词：
D O I：
10.1016/0165-2427(90)90108-5
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Nine stable hybridoma cell lines were established which secreted specific monoclonal antibodies (MAbs) to bovine gamma-interferon (BoIFN-γ). Specific binding of each of the MAbs to recombinant BoIFN-γ (rBoIFN-γ) was demonstrated in an indirect ELISA, whilst none of the MAbs bound to rBoIFN-α or rBoIFN-β. In a Western blot the MAbs reacted with the 16 kDa and 32 kDa polypeptides present in rBoIFN-γ preparations. Competitive ELISA's showed that four MAbs bound to one epitope on rBoIFN-γ, and the other five MAbs bound to a separate epitope. Two MAbs, each recognising different epitopes, were shown to neutralise the anti-viral activity of natural BoIFN-γ. © 1990.
引用
收藏
页码：37 / 46
页数：10
相关论文
共 50 条
  • [1] MONOCLONAL-ANTIBODIES TO NATURAL HUMAN GAMMA-INTERFERON
    NAGIEVA, FG
    BARKOVA, EP
    ANDZHAPARIDZE, OG
    [J]. VOPROSY VIRUSOLOGII, 1991, 36 (04) : 306 - 309
  • [2] MONOCLONAL-ANTIBODIES AGAINST MURINE GAMMA-INTERFERON
    PRAT, M
    GRIBAUDO, G
    COMOGLIO, PM
    CAVALLO, G
    LANDOLFO, S
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (14): : 4515 - 4519
  • [3] THE PURIFICATION AND CHARACTERIZATION OF RAT GAMMA-INTERFERON BY USE OF 2 MONOCLONAL-ANTIBODIES
    VANDERMEIDE, PH
    DUBBELD, M
    VIJVERBERG, K
    KOS, T
    SCHELLEKENS, H
    [J]. JOURNAL OF GENERAL VIROLOGY, 1986, 67 : 1059 - 1071
  • [4] PRODUCTION AND CHARACTERIZATION OF SPECIFIC BOVINE PLASMINOGEN MONOCLONAL-ANTIBODIES
    DUPONT, D
    LABIAU, S
    GROSCLAUDE, J
    COLLIN, JC
    [J]. HYBRIDOMA, 1994, 13 (06): : 543 - 547
  • [5] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR BOVINE OVIDUCTAL GLYCOPROTEINS
    ABE, H
    NUMAZAWA, C
    ABE, M
    HOSHI, H
    OIKAWA, T
    [J]. JOURNAL OF EXPERIMENTAL ZOOLOGY, 1993, 265 (01): : 77 - 83
  • [6] DETECTION OF HYBRIDOMAS SECRETING MONOCLONAL-ANTIBODIES TO HUMAN GAMMA-INTERFERON USING A RAPID SCREENING TECHNIQUE AND SPECIFICITY OF CERTAIN MONOCLONAL-ANTIBODIES TO GAMMA INTERFERON
    MEAGER, A
    PARTI, S
    BARWICK, S
    SPRAGG, J
    OHAGAN, K
    [J]. JOURNAL OF INTERFERON RESEARCH, 1984, 4 (04): : 619 - 625
  • [7] USE OF MONOCLONAL-ANTIBODIES AS SENSITIVE AND SPECIFIC PROBES FOR BIOLOGICALLY-ACTIVE HUMAN GAMMA-INTERFERON
    CHANG, TW
    MCKINNEY, S
    LIU, V
    KUNG, PC
    VILCEK, J
    LE, JM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (16): : 5219 - 5222
  • [8] EPITOPE MAPPING OF RECOMBINANT HUMAN GAMMA-INTERFERON USING MONOCLONAL-ANTIBODIES
    FAVRE, C
    WIJDENES, J
    CABRILLAT, H
    DJOSSOU, O
    BANCHEREAU, J
    DEVRIES, JE
    [J]. MOLECULAR IMMUNOLOGY, 1989, 26 (01) : 17 - 25
  • [9] HUMAN GAMMA-INTERFERON PRODUCTION BY LEUKOCYTES INDUCED WITH MONOCLONAL-ANTIBODIES RECOGNIZING T-CELLS
    VONWUSSOW, P
    PLATSOUCAS, CD
    WIRANOWSKASTEWART, M
    STEWART, WE
    [J]. JOURNAL OF IMMUNOLOGY, 1981, 127 (03): : 1197 - 1200
  • [10] CHARACTERIZATION OF MONOCLONAL-ANTIBODIES DEVELOPED TO BOVINE LEUKOCYTE INTERFERON
    STRECK, BJ
    DON, G
    BENTON, CV
    [J]. HYBRIDOMA, 1983, 2 (01): : 124 - 124
12345