METHYL 3-AMINO-3-DEOXY-BETA-D-GALACTOPYRANOSYL-(1-]4)-2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE - AN INHIBITOR OF UDP-D-GALACTOSE-BETA-D-GALACTOPYRANOSYL-(1-]4)-2-ACETAMIDO-2-DEOXY-D-GLUCOSE (1-]3)-ALPHA-D-GALACTOPYRANOSYLTRANSFERASE

被引：16
|
作者：
HELLAND, AC
HINDSGAUL, O
PALCIC, MM
STULTS, CLM
MACHER, BA
机构：
[1] UNIV ALBERTA,DEPT CHEM,EDMONTON,AB T6G 2G2,CANADA
[2] SAN FRANCISCO STATE UNIV,DEPT CHEM & BIOCHEM,SAN FRANCISCO,CA 94132
来源：
CARBOHYDRATE RESEARCH | 1995年 / 276卷 / 01期
关键词：
UDP-D-GALACTOSE; BETA-D-GALACTOPYRANOSYL-(1-]4)-2-ACETAMIDO-2-DEOXY-D-GLUCOSE (1-]3)-ALPHA-D-GALACTOPYRANOSYLTRANSFERASE; ALPHA-(1-]3)-GALOCTOSYLTRANSFERASE; EC; 2.4.1.151; ENZYME INHIBITOR; METHYL 3-AMINO-3-DEOXY-BETA-D-GALACTOPYRANOSYL-(1-]4)-2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE;
D O I：
10.1016/0008-6215(95)00165-P
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
UDP-D-galactose: beta-D-galactopyranosyl-(1 --> 4)-2-acetamido-2-deoxy-D-glucose alpha(1 --> 3)-D-galactopyranosyltransferase [E.C. 2.4.1.151] transfers D-galactosyl-residues from the sugar nucleotide with retention of configuration. We report here that synthetic methyl 3'-amino-3'-deoxy-N-acetyllactosaminide (9), where the hydroxyl group normally undergoing galactosylation has been replaced by amino group, is an inhibitor for this enzyme with K-i = 104 mu M. The mode of inhibition is not competitive, but appears to be specific, since other glycosyltransferases were not affected by 9.
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页码：91 / 98
页数：8
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