PYRIDYLOXOBUTYL DNA ADDUCTS INHIBIT THE REPAIR OF O(6)-METHYLGUANINE

The carcinogenic tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK), both methylates and pyridyloxobutylates DNA. O6-Methylguanine (O6-mG) persistence has been correlated to NNK-induced lung tumor formation in A/J mice. The pyridyloxobutylation pathway enhances the tumorigenicity of the methylation pathway. In this paper we test the hypothesis that DNA pyridyloxobutylation increases O6-mG persistence by inhibiting the repair protein O6-alkylguanine-DNA alkyltransferase (AGT). Pyridyloxobutylated DNA was generated by reacting calf thymus DNA with the model pyridyloxobutylating agent 4-(acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone (NNKOAc) in the presence of esterase. The alkylated DNA inhibited the ability or partially purified rat liver AGT to repair O6-mG when it was incubated with AGT prior to the addition of H-3-methylated DNA. The extent of inhibition was dependent on the amount of NNKOAc reacted with DNA. The ability of NNKOAc-treated DNA to inhibit AGT was destroyed when the DNA was subjected to neutral thermal hydrolysis. Approximately 1 pmol of AGT was inhibited for every 25 to 50 pmol of 4-hydroxy-1-(3-pyridyl)-1-butanone-releasing adducts present in NNKOAc-treated DNA. The inhibitory activity of this alkylated DNA was relatively stable under physiological conditions (pH 7.4, 37-degrees-C). Only 13% of the AGT reactive activity was lost after 7 days. When pyridyloxobutylated DNA was incubated simultaneously with H-3-methylated DNA and AGT, a significant reduction in [H-3]methyl transfer to AGT was observed. The levels of reduction were similar to those observed when unlabeled methylated DNA containing comparable levels of O6-mG was substituted for NNKOAc-treated DNA. Based on these results, a cocarcinogenic role for pyridyloxobutylation in NNK-induced lung tumorigenesis is proposed in which pyridyloxobutyl DNA adduct(s) compete with O6-mG for reaction with AGT resulting in sustained levels of O6-mG. These enhanced levels then increase the probability of tumor induction by NNK.