STATISTICAL-MECHANICAL MODEL OF PROTEIN PRECIPITATION BY NONIONIC POLYMER

被引：85

作者：

MAHADEVAN, H ^{[1
]}

HALL, CK ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,DEPT CHEM ENGN,RALEIGH,NC 27695

来源：

AICHE JOURNAL | 1990年 / 36卷 / 10期

关键词：

D O I：

10.1002/aic.690361007

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

A theoretical approach to predict the solubility of proteins in solutions containing nonionic polymers is presented. The effective protein‐protein interaction due to the presence of the polymer is related to the volume‐exclusion potential of Asakura and Oosawa. Statistical‐mechanical perturbation theory, as originally applied by Gast et al. to model colloidal flocculation, is used to calculate free energies, from which solubility curves for varying protein‐polymer diameter ratios are obtained. The theory correctly predicts all the trends observed in experimental studies of these systems. To explain the influence of processes parameters such as the pH and the ionic strength on protein solubility, the intermolecular potential is improved by the addition of an electrostatic interaction term. It is found that theoretical predictions of the variation in protein solubility, both with the solution pH and the ionic strength, are in accordance with experimental observations. Copyright © 1990 American Institute of Chemical Engineers

引用

页码：1517 / 1528

页数：12

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