REGULATION OF GLUTATHIONE SYNTHESIS IN LEAVES OF TRANSGENIC POPLAR (POPULUS-TREMULA X POPULUS-ALBA) OVEREXPRESSING GLUTATHIONE SYNTHETASE

被引：155
|
作者：
STROHM, M
JOUANIN, L
KUNERT, KJ
PRUVOST, C
POLLE, A
FOYER, CH
RENNENBERG, H
机构：
[1] UNIV FREIBURG,INST FORSTBOT & BAUMPHYSIOL,D-79085 FREIBURG,GERMANY
[2] INRA,METAB LAB,F-78026 VERSAILLES,FRANCE
[3] AECI LTD,DEPT RES & DEV,MODDERFONTEIN 1645,SOUTH AFRICA
[4] INRA,BIOL CELLULAIRE LAB,F-78026 VERSAILLES,FRANCE
来源：
PLANT JOURNAL | 1995年 / 7卷 / 01期
关键词：
D O I：
10.1046/j.1365-313X.1995.07010141.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The poplar hybrid Popolus tremula x P. alba was transformed with the Escherichia coli gene for glutathione synthetase (gsh II) targetted to the cytosol. Leaves of five lines of transgenic plants exhibited glutathione synthetase activities 15- to 60-fold higher than those of wild-type plants. Total glutathione levels and GSH/GSSG ratios were similar in transgenic and wild-type plants. Precursor feeding experiments with cysteine and gamma-glutamylcysteine suggest that glutathione synthesis in the cytoplasm is controlled by a multistep procedure that includes (i) the availability of cysteine, (ii) the availability of gamma-glutamylcysteine, and (iii) regulation of the activities of both gamma-glutamylcysteine synthetase and glutathione synthetase. However step (ii) may set an upper limit for the cellular glutathione content.
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页码：141 / 145
页数：5
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