NEUTROPHIL ANTIBACTERIAL ACTIVITY IN PATIENTS WITH CHRONIC-BRONCHITIS - THE USE OF PROINFLAMMATORY CYTOKINES AND ALLOGENEIC LYMPHOCYTES FOR IMMUNOCORRECTION IN-VITRO

In patients with acute and chronic bronchitis descreased neutrophil phagocytic, induced O2-dependent metabolic, bactericidal activity and the content of lysosomal cationic proteins, increased spontaneous O2-dependent metabolic activity were revealed. The most pronounced neutrophil functional disorders were detected in patients with chronic obstructive bronchitis (COB). The use of human immunoregulatory recombinant cytokines (interleukin-1beta, interferon-gamma, tumor necrosis factor alpha) failed to augment in vitro suppressed neutrophil antibacterial (phagocytic) activity in patients with COB. These patient's neutrophil antibacterial activity restored in vitro in the presence of allogeneic (nonactivated or interleukin-2-activated) lymphocytes. The data obtained provide evidence for healthy donor's lymphocytes use in immunocorrection of patients with chronic bronchitis.