ION-EXCHANGE CHROMATOGRAPHY WITH A SOFT SORBENT OPERATING IN A PRESSURIZED COLUMN

被引：5
|
作者：
BARU, MB [1 ]
KOZLOVSKYVAGENINA, IV [1 ]
机构：
[1] RUSSIAN ACAD SCI,SHEMYAKIN INST BIOORGAN CHEM,PUSHCHINO 142292,RUSSIA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1993年 / 657卷 / 01期
关键词：
D O I：
10.1016/0021-9673(93)83052-T
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A standard Pharmacia column was modified to enable pressurization with inert gas. The column moveable adaptor can follow the gel as the volume changes under pressure; thus, ''dead volume-free'' operation is achieved. A trial separation of natural bee venom components was carried out on the modified column with CM-Sephadex C,, in a pH and ionic strength gradient. It was found that, at 54 kPa pressure, the elution times of the bee venom constituents phospholipase a,, apamin and melittin are reduced and the resolution is improved.
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页码：199 / 203
页数：5
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